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    poly-d-lysine substrate coating allows interaction with ______ sites on cells

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    get poly-d-lysine substrate coating allows interaction with ______ sites on cells from screen.

    Poly

    Poly-D (or L)-lysine coated surfaces promote attachment and growth of various cell types under serum free and/or low serum conditions.

    POLY-D (OR L)-LYSINE

    POLY-D (OR L)-LYSINE Poly-D (or L)-Lysine coated surface promotes attachment and growth of various cell types under serum free and/or low serum conditions.

    is a synthetic positively charged polymer, existing as two enantiomers, . Both are commonly used however poly-D-lysine is not degraded by cellular proteases and is therefore often the preferred choice. As poly-D (or L)-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants.

    is useful in promoting cell adhesion to solid substrates and enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface.

    When adsorbed to the polystyrene surface, increases the number of positively-charged sites available for cell binding. This uniform net positive charge is preferred by certain cell types and can subsequently enhance cell attachment, growth and differentiation of these cell types, especially under serum-free and/or low serum conditions.

    often improve attachment and growth of primary neurons, glial cells, neuroblastomas, and a variety of transfected cell lines. In addition, these coated surfaces are often used to reduce cell detachment that often occurs during multiple washing steps that are associated with cell-based assays.

    see Test for cell proliferation and viability and cell morphology and differentation on poly-D-lys coated plates

    Biomat Poly-D (or L)-Lysine Coated products

    This is the available configuration of Poly-D (or L)-Lysine Coated Plates products by Biomat.

    Poly-d-lysine Coated 96 Well Plates

    Poly-l-lysine Coated 96 Well Plates

    Poly-d-lysine Coated 384 Well Plates

    Poly-L-lysine Coated 384 Well Plates

    Other Tissue Culture Plates

    Poly-D (or L)-Lysine Coated Surfaces main features

    Biomat’s Poly-D (or L)-Lysine Coated 96-Well plates have the following characteristics:

    Ready to use

    Manufactured under ISO:9001 guidelines

    Sterile

    Lid present on each single plate

    Certificate of Quality is released for every lot

    For Research Use Only

    Poly-D (or L)-Lysine Coated Surfaces Product Specifications

    Coating

    Poly-D (or L)-Lysine (M.W. 70-150 kDa)

    Stability

    The microplates, if unopened, are sterile and stable refrigerated until the expiration date printed on the label.

    Other Biomat Microplates:

    Biomat 96 Well Plates

    Biomat HTS Plates

    Biomat Tissue Culture Plates

    Biomat Immunotoxicity plates

    Biomat Mouse Monoclonal Antibodies Isotyping Plates

    Biomat Anti Tag Plates

    DOWNLOADS and TECHNICAL DATA

    DOWNLOAD FILE

    SEE THE RELATED PRODUCTS

    STANDARD SURFACES:

    High binding capacityMedium binding capacityNo binding capacity

    FEATURED COATED SURFACES:

    Aminated (primary amine)BiotinCalmodulinCarboxylatedConcanavalin AHeparin catcherCoated surfaces for Immunotoxicity ELISA testJacalinMab Anti TagMaleimideMouse Monoclonal Antibodies Isotyping ELISANeutravidinPoly-D (or L)-LysinePoly-L-ArgininePoly-L-LysineProtein AProtein A/GProtein GSecondary AntibodiesStreptavidinStreptavidin High BindingWheat germ

    स्रोत : www.biomat.it

    Poly

    Poly-D-Lysine solution, 1.0 mg/mL The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel. - Find MSDS or SDS, a COA, data sheets and more information.

    A-003-E Sigma-AldrichPoly-D-Lysine solution, 1.0 mg/mL

    The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.

    Poly-D-Lysine solution, 1.0 mg/mL: Malzeme Güvenlik Bilgi Formu (MSDS) veya SDS, Analiz Sertifikası (COA) ve Kalite Uygunluk Sertifikası (COQ), dosyalar, broşürler ve diğer dokümanlar.

    SDS

    Key Applications: Cell Culture

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    « »

    Overview

    Key Spec Table

    Key Applications CULT Description

    Catalogue Number A-003-E

    Trade Name Specialty Media

    Description Poly-D-Lysine solution, 1.0 mg/mL

    Overview Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.

    Alternate Names poly lysine Product Information

    Presentation Liquid. 20ml in sterile water.

    Quality Level MQ100 Applications

    Application The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.

    Key Applications Cell Culture

    Application Notes The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.

    Surface area of plates: (pi)(Radius squared) in cm(2)

    In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.

    Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.

    Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.

    Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.

    Use plates immediately or store with PBS for up to 5 days.

    Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.

    Biological Information

    Media Form Liquid Stem Cell Type

    Human Embryonic Stem Cells

    Mesenchymal Stem Cells

    Neural Stem Cells

    Hematopoietic Stem Cells

    Epithelial Cells

    Pancreatic Stem Cells

    Induced Pluripotent Stem Cells

    Product Usage Statements

    Usage Statement

    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

    Storage and Shipping Information

    Storage Conditions Store at -20C for upto 18 months.

    Packaging Information

    Material Size 20 mL

    Recently Viewed

    A-003-E Sigma-Aldrich Poly-D-Lysine... « »

    स्रोत : www.merckmillipore.com

    Poly

    Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

    Poly-lysine | Poly-D-Lysine | Poly-L-Lysine

    Poly-lysine | Poly-D-Lysine | Poly-L-Lysine

    George Sitterley

    Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components. While some cells are able to synthesize these components, others require an exogenous source, particularly when grown in serum-free culture. To help facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of your cells, We offer a number of sterile, cell culture tested polyamino acids. In histochemical applications both polymers of D- and L-lysine are used to coat slides to promote attachment of cells. Poly-L-lysine has also been reported to improve protein coating of ELISA plates.

    We offer both Poly-D-Lysine and Poly-L-Lysine in several molecular weight ranges. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

    Poly-L-Lysine is a charge enhancer; therefore, it can be used for coating many surfaces.

    Poly-lysines

    Name Form Molecular

    Weight Conc. Storage

    Temp. Application Cat.

    No.

    Poly-L-lysine sterile-filtered 70,000-150,000 0.01% 2-8°C Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P4707

    Poly-Lornithine sterile-filtered sterile-filtered 30,000-70,000 0.01% 2-8°C Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. P4957

    Poly-L-lysine sterile-filtered 150,000-300,000 0.01% 2-8°C Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P4832

    Poly-D-lysine lyophilized powder, γ-irradiated average mol wt 30,000-70,000 - −20°C Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates P7280

    Poly-L-lysine lyophilized powder, γ-irradiated 30,000-70,000 - −20°C Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P9155

    Poly-D-lysine lyophilized powder, γ-irradiated 70,000-150,000 - −20°C Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates. P6407

    Poly-L-lysine lyophilized powder, γ-irradiated 70,000-150,000 - −20°C Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P6282

    Poly-D-lysine lyophilized powder, γ-irradiated >300,000 - −20°C Poly-lysine with mol. wt. >30,000 is useful in promoting cell adhesion to solid substrates. P7405

    Poly-L-lysine lyophilized powder, γ-irradiated ≥300,000 - −20°C Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application. P5899

    Materials

    Product No. Description SDS Pricing

    P6407 Poly-D-lysine hydrobromidemol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

    P7280 Poly-D-lysine hydrobromideaverage mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

    P7405 Poly-D-lysine hydrobromidemol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

    P6282 Poly-L-lysine hydrobromidemol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

    P9155 Poly-L-lysine hydrobromidemol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

    P5899 Poly-L-lysine hydrobromidemol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

    P4707 Poly-L-lysine solution0.01%, sterile-filtered, BioReagent, suitable for cell culture

    P4832 Poly-L-lysine solutionmol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

    P4957 Poly-L-ornithine solutionmol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

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