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    to prepare a temporary mount to observe pollen germination

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    get to prepare a temporary mount to observe pollen germination from screen.

    Study Pollen Germination On A Slide

    To study pollen germination on a slide using a microscope. Explore the technique and correct procedure required to perform this experiment

    CBSECBSE Class 12CBSE Class 12 Biology PracticalStudy Pollen Germination On A Slide

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    Study Pollen Germination On A Slide

    Study Pollen Germination On A Slide Aim

    To study pollen germination on a slide.

    Necessary Materials & Apparatus

    Freshly plucked seasonal flowers, beaker, boric acid, sucrose, microscope and cavity slide.

    Procedure

    The first step involves the preparation of a nutrient solution. This is done by dissolving 10g of sucrose as well as 10mg of boric acid in 100ml of water.

    Pour a few drops of this solution onto the cavity slide. Then, use a brush or fingers to gently dust a few pollen grains from the stamen of mature flowers.

    Let the slide set for 5 mins. Then, use the microscope to view the slides in 30-minute intervals.

    Observation

    The pollen grains will germinate when submerged in the nutrient-rich medium. This is characterized by the enlargement of the vegetative/tube cell. It emerges through one of the germ pores, eventually forming a pollen tube. The generative cell nucleus grows into the pollen tube and makes two male gametes (sperm nuclei). The male gamete is either spherical or lenticular in outline.

    Precautions

    Ensure that the flowers are freshly picked

    The observation slide should be a cavity slide, meaning that it has a depression in the centre.

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    Study of Pollen Germination on a Slide

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    Introduction

    Last updated date: 17th Jan 2023

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    In this article, students will learn about the germination of pollen on a slide in the form of an experiment but first, let's understand the concept of pollen germination. Pollination is the method by which pollen grains are transferred from the anther of a flower which is the male part to the stigma of the same or different flower which is the female part of the flower. Pollination is mostly carried out by abiotic components of the environment such as the wind and the water.

    Pollen grains germinate when they absorb nutrients and water and grow a pollen tube from the style to the ovary part through one of the germ pores. Then the nucleus moves down to the pollen tube as well as the generative cells, which in turn develop into two male gametes. One of the two sperm fuses with the egg cell present in the ovule and develops into the embryo, while the other combines with another nucleus from the ovule and forms the endosperm. This is called double fertilisation. This is what happens during pollen germination. Now let's study the process of pollen germination when studied in a slide.

    Aim

    The experiment aims to observe pollen germination on a slide.

    Materials Required

    Freshly plucked flower from grass or china rose,

    10 mg boric acid Beaker Microscope Sucrose (10 g) Coverslips Slide Dropper

    Magnesium sulphate (20 mg)

    Calcium nitrate (30 mg)

    Procedure

    The first step is to prepare the nutrient solution by dissolving the sucrose and boric acid in water. Then this mixture has to be dropped using a dropped on the cavity slip. Then using a brush or simply by fingers, brush off a few pollen grains and let the slide for 15 minutes. Then the microscope has to be used to observe the slide at intervals of 30 minutes.

    Observations

    It can be observed that by enlargement of the vegetative cells, the pollen grains are germinating when submerged in the nutrient medium. A pollen tube is formed when the nucleus grows and emerges from one of the germ pores and forms two male gametes which can be either spherical or lenticular in shape. These pollen grains that germinate are called viable pollen grains. These gametes are called sperm nuclei. There will also be some pollen grains that will not form pollen tubes, hence, they are called non-viable pollen grains.

    Precautions

    There are precautions to be observed while performing this experiment:

    Dirty or previously used slides should not be used for dusting pollen grains. Slides should be clean and dry for use.

    Only a few drops of the nutrient medium is required for germination. 2-3 drops of the solution are enough, more than that may hinder the process of pollen germination.

    Only freshly plucked flowers should be used and the experiment should be conducted within some time of plucking.

    The cavity slide used for observation should have depression at the centre so that the solution can be held in that cavity and does not flow.

    Conclusion

    Pollen germination can be carried out in a nutrient medium and studied under a microscope when proper environmental conditions are given.

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    FAQs on Study of Pollen Germination on a Slide

    1. Why Do Some Pollen Grains Fail to Germinate?

    Even during the experiment to study pollen germination on a slide, you will observe that some pollen grains fail to germinate. Sometimes the nutrient mixture fails to do the job and in real life when pollen doesn't germinate, it’s because sometimes when the grain falls on the stigma it does not get the right conditions to carry out germination. To study pollen germination on a slide correctly remember, all pollen tubes don't have the same length and the newly developed ones have smaller tubes, unlike the old ones which are longer in size. The tube will not reach the stigma if not germinated.

    2. Why Do We Use Sucrose as a Medium for Pollen Germination?

    The natural process allows the pollen germination only on the compatible stigma. It is the stigma that stimulates the germination process by producing a sugary liquid. The study of pollen germination on a slide is the same because sucrose fulfills multiple criteria that are important to carry out the pollen germination such as a substrate for the pollen metabolism and required osmotic pressure. Therefore, to study pollen germination on a slide, you will notice sucrose produces the right tube growth in vitro for pollen germination. This is the precise reason why we use sucrose as a medium for pollen germination.

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    Class XII CBSE Biology Practical Study Pollen Germination on a Slide

    Spread the loveAim:  To study pollen germination on a slide. Apparatus and materials required: Fresh seasonal flower slides, microscope, beaker, cavity slide, boric acid, sucrose. Procedure: Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water. Take a few drops of this solution on a clear slide, dust … Continue reading "Study Pollen Germination on a Slide"

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    OCTOBER 1, 2017 BY ADMIN

    Study Pollen Germination on a Slide

    Spread the love

    Aim:  To study pollen germination on a slide.Apparatus and materials required: Fresh seasonal flower slides, microscope, beaker, cavity slide, boric acid, sucrose.Procedure:

    Prepare a nutrient solution by dissolving 10g sucrose and 10 mg boric acid in 100 ml water.

    Take a few drops of this solution on a clear slide, dust a few pollen grains from the stamen of a mature flower in it.

    Obverse the slide under the microscope after 5 minutes. Keep observing the slide regularly after intervals of half an hour.

    Observations:

    In a nutrient solution or nutrient-rich medium, the pollen grains germinate. The tube/vegetative cell enlarges and comes out the pollen grains through one of the germ pores to form a pollen tube. The generative cell nucleus soon passes into it and dives to make 2 sperm nuclei (male gametes). Each male gamete is lenticular to spherical in outline.

    Precautions:

    Flowers should be freshly picked

    Use a clear cavity slide to observe pollen grain

    You can also get Class XII Practicals on Biology, Physics, and Physical Education.

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    33 Replies to “Study Pollen Germination on a Slide”

    Please post the clear pictures please

    Reply Very good Reply nice Reply Like Reply Not bad Reply

    Also give result and conclusion of the experiment

    Reply Not bad Reply NOT YET Reply Nice explained Reply nice Reply Conclusion? Reply its very nice Reply

    What is the principle of in vitro pollen germination …?

    Reply its too helpful Reply

    Is the figure right???

    Reply

    Hey rashan it a mahi I need your help

    Reply I think yes… Reply Hoooo Reply I mean noo Reply I mean noo Reply

    Arshan I will support you..

    Reply

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    Please give the conclusion

    Reply Thanks Reply

    Plz give the clear pic

    Reply

    Send me plz clear picture

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    Mohammed 8 day ago
    4

    Guys, does anyone know the answer?

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