write the name of chemical test when protein in food stuff is treated with concentrated hno3

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Xanthoproteic reaction

Xanthoproteic reaction

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Product of xanthoproteic reaction with the characteristic yellow color

The xanthoproteic reaction is a method that can be used to detect a presence of protein soluble in a solution, using concentrated nitric acid. The test gives a positive result in amino acids carrying aromatic groups, especially in the presence of tyrosine. If the test is positive the proof is neutralized with an alkali, turning dark yellow. The yellow colour is due to xanthoproteic acid which is formed due to nitration of certain amino acids, most common examples being tyrosine and tryptophan.[1] This chemical reaction is a qualitative test, determining the presence or absence of proteins.

Reaction of nitration of tyrosine as an example xanthoproteic reaction

Procedure[edit]

Add 1 ml of concentrated HNO3 to 1 ml of the test sample. Heat the mixture and cool it. Slowly add sodium hydroxide (40% w/v in water) solution until the mixture becomes alkaline and a colour change is noted. If the colour changes from yellow to orange, this indicates the presence of an aromatic amino acid.

When human skin or nails are exposed to nitric acid, they turn yellow after some time, indicating the presence of protein. The finger nails show a bright yellow colour (finger nails are made up of keratin, which is a protein) which cannot be scraped off, unlike the yellow colouration on the skin which can be peeled off.[]

See also[edit]

Canary Girls Xanthoproteic acid

References[edit]

^ Chatterjea (1 January 2004). . Jaypee Brothers Publishers. p. 51. ISBN 978-81-8061-204-6.

Categories: Biochemistry detection reactionsProtein methods

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Proteins when heated with conc. HNO3 give yellow colour. This is known as?

Click here👆to get an answer to your question ✍️ Proteins when heated with conc. HNO3 give yellow colour. This is known as?

Proteins when heated with conc. HNO

Question 3 ​

give yellow colour. This is known as?

A

Hoppe's test

B

Acid-base test

C

Biuret's test

D

Xanthoprotic test

Medium Open in App

Updated on : 2022-09-05

Solution Verified by Toppr

Correct option is D)

Xanthoproteic test—When a protein is treated with conc. H2SO4, a yellow colour is produced. This test is given by a protein which consists of α-amino-acids containing a benzene ring such as tyrosine, phenylalanine etc.

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On heating with conc. HNO3, proteins give yellow colour. This test is called

Formation of a yellow colour, when a protein is heated with conc. HNO(3) is called Xanthoproteic test.

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On heating with conc. HNO_3, p...

On heating with conc. `HNO_3`, proteins give yellow colour. This test is called

Updated On: 27-06-2022

( 00 : 20 ) ADVERTISEMENT Text Solution Open Answer in App A oxidising test B Xanthoproteic test C Hoppe's test D Acid base test. Answer

The correct Answer is B

Solution

Formation of a yellow colour, when a protein is heated with conc. `HNO_(3)` is called Xanthoproteic test.

Answer

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